correlation of adiponectin mrna abundance and its receptors with quantitative parameters of sperm motility in rams
Authors
abstract
objective: adiponectin and its receptors (adipor1 and adipor2), known as adiponectin system, have some proven roles in the fat and glucose metabolisms. several studies have shown that adiponectin can be considered as a candidate in linking metabolism to testicular function. in this regard, we evaluated the correlation between sperm mrna abundance of adiponectin and its receptors, with sperm motility indices in the present study. materials and methods: in this completely randomized design study, semen samples from 6 adult rams were fractionated on a two layer discontinuous percoll gradient into high and low motile sperm cells, then quantitative parameters of sperm motility were determined by computer-assisted sperm analyzer (casa). the mrna abundance levels of adiponectin, adipor1 and adipor2 were measured quantitatively using real-time reverse transcriptase polymerase chain reaction (qrt-pcr) in the high and low motile groups. results: firstly, we showed that adiponectin and its receptors (adipor1 and adipor2) were transcriptionally expressed in the ram sperm cells. using pfaff based method qrtpcr, these levels of transcription were significantly higher in the high motile rather than low motile samples. this increase was 3.5, 3.6 and 2.5 fold change rate for adiponectin, adipor1 and adipor2, respectively. some of sperm motility indices [curvilinear velocity (vcl), straight-line velocity (vsl), average path velocity (vap), linearity (lin), wobble (wob) and straightness (str)] were also significantly correlated with adiponectin and adipor1 relative expression. the correlation of adipor2 was also significant with the mentioned parameters, although this correlation was not comparable with adiponectin and adipor1. conclusion: this study revealed the novel association of adiponectin system with sperm motility. the results of our study suggested that adiponectin is one of the possible factors which can be evaluated and studied in male infertility disorders.
similar resources
Correlation of Adiponectin mRNA Abundance and Its Receptors with Quantitative Parameters of Sperm Motility in Rams
Objective Adiponectin and its receptors (AdipoR1 and AdipoR2), known as adiponectin system, have some proven roles in the fat and glucose metabolisms. Several studies have shown that adiponectin can be considered as a candidate in linking metabolism to testicular function. In this regard, we evaluated the correlation between sperm mRNA abundance of adiponectin and its receptors, with sperm moti...
full textCorrelation of Adiponectin mRNA Abundance and Its Receptors with Quantitative Parameters of Sperm Motility in Rams
BACKGROUND Adiponectin and its receptors (AdipoR1 and AdipoR2), known as adiponectin system, have some proven roles in the fat and glucose metabolisms. Several studies have shown that adiponectin can be considered as a candidate in linking metabolism to testicular function. In this regard, we evaluated the correlation between sperm mRNA abundance of adiponectin and its receptors, with sperm mot...
full textAdiponectin Effect on The Viability of Human Endometrial Stromal Cells and mRNA Expression of Adiponectin Receptors
full text
the study of aaag repeat polymorphism in promoter of errg gene and its association with the risk of breast cancer in isfahan region
چکیده: سرطان پستان دومین عامل مرگ مرتبط با سرطان در خانم ها است. از آنجا که سرطان پستان یک تومور وابسته به هورمون است، می تواند توسط وضعیت هورمون های استروئیدی شامل استروژن و پروژسترون تنظیم شود. استروژن نقش مهمی در توسعه و پیشرفت سرطان پستان ایفا می کند و تاثیر خود را روی بیان ژن های هدف از طریق گیرنده های استروژن اعمال می کند. اما گروه دیگری از گیرنده های هسته ای به نام گیرنده های مرتبط به ا...
15 صفحه اولPeroxisome proliferator-activated receptors (PPARα, PPARγ and PPARβ/δ) gene expression profile on ram spermatozoa and their relation to the sperm motility
Peroxisome proliferator-activated receptors (PPARs) are a member of nuclear receptors superfamily, which mainly regulate the expression of target genes involved in lipid and energy metabolism. These receptors are divided to three isotypes: PPARα, PPARγ and PPARβ/δ. Each isotype has a distinct tissue distribution relating to the distinct functions. In this study, the mRNA abundance for PPARα, PP...
full textmRNA Expression of VEGF and Its Receptors in Fallopian Tubes of Women with Ectopic Pregnancies
Background Establishment of viable pregnancy requires embryo implantation and placentation. Ectopic pregnancy (EP) is a pregnancy complication which occurs when an embryo implants outside of the uterine cavity, most often in a fallopian tube. On the other hand, an important aspect of successful implantation is angiogenesis. Vascular endothelial growth factor (VEGF) is a potent angiogenic factor...
full textMy Resources
Save resource for easier access later
Journal title:
international journal of fertility and sterilityجلد ۱۰، شماره ۱، صفحات ۱۲۷-۱۳۵
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023